Fiber Tracking

Single Fiber

This method allows the semiautomatic tracking of the length of  fluorescently labeled DNA fiber tracks in two channels.

Usage

Using the multi point tool define the start and end points of the labeled tracks. Starting the "Single fiber" plugin, the intensity based algorithm will track the fiber and record the respective track length in the results table.

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  • Dually labeled DNA strands with the tracked fiber
  • Dually labeled DNA strands with marked start and end points
  • Dually labeled DNA strand

Dual Fiber

This method allows the semiautomatic tracking of the length of  fluorescently labeled DNA fiber tracks in two channels. In contrast, to the "Single fiber" plugin a second switch of labeling can be measured.

Usage

Using the multi point tool define the start, switch and end points of the labeled tracks. Starting the "Dual fiber" plugin, the intensity based algorithm will track the fiber and record the respective track length in the results table.

    >
  • Dually labeled DNA strands with the tracked fiber
  • Dually labeled DNA strands with marked start, switch and end points
  • Dually labeled DNA strands