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Responsibilities

Affiliated with the Department of Chemistry

Project: In vitro evaluation of the molecular toxicity of carvacrol using the enzyme-modified Fluorimetric detection of Alkaline DNA Unwinding (emFADU)

_{This research is aimed at evaluating the molecular cytotoxicity of carvacrol, a component of crude plant essential oils that have been observed to demonstrate appreciable antioxidant capacity in the storage of lipid-rich foods compared to butylated hydroxytoluene (BHT). For the proposed research, the genotoxicity, apoptosis induction, capacity to induce DNA damage in plasmid DNA, and the induction of DNA strand breaks by carvacrol will be compared to an established genotoxin and BHT. The apoptosis induction will be studied using Annexin V APC/PI flow cytometry. The detection of the DNA strand breaks and plasmid DNA damage will be studied using the enzyme-modified version of cell-based Fluorimetric detection of Alkaline DNA Unwinding (emFADU). The results of this research are important as they will shed light on the safety of carvacrol proposed as a natural alternative to synthetic antioxidant food additives.}

ZUKOnnect Fellow from 07/2023

Responsibilities

Doctoral Candidates