Instruments at FlowKon
The Core Facility for Flow Cytometry and Cell Sorting FlowKon currently hosts six high-end instruments supporting the use of a wide range of flow cytometry applications.
Analyzer Flow Cytometry
We provide access to four analyzers which are equipped with up to 5 lasers allowing for the simultaneous detection of up to 18 parameters within one cell.
Cytometer | Laser | Parameter |
|---|---|---|
BD LSRFortessa™ | 5 Laser (355, 405, 488, 561, 633 nm) | ≤ 18 Parameter |
| Beckman Coulter CytoFLEX™ | 5 Laser (375, 405, 488, 561, 638 nm) | ≤ 18 Parameter |
| BD FACSVerse™ | 3 Laser (405, 488, 633 nm) | ≤ 10 Parameter |
| BD FACSLyric™ | 3 Laser (405, 488, 633 nm) | ≤ 12 Parameter |
Technical details and laser configuration of Analyzer Flow Cytometry.
Sorter MACS & FACS
The Miltenyi Biotec autoMACS® Pro Separator and the BD FACSAria™ IIIu/III present two different cell sorting techniques opening the chance to physically separate target cells from a bulk cell populations.
Cell Sorter | Laser | Parameter |
|---|---|---|
| BD FACSAria™ IIIu | 4 Laser (405, 488, 561, 633nm) | ≤ 12 Parameter |
| BD FACSAria™ III | 5 Laser (375/405, 488, 561, 633nm) | ≤ 12 Parameter |
Technical details and laser configuration of BD FACSAria™ IIIu and BD FACSAria™ III
Cell Sorter | Properties | |
|---|---|---|
| Miltenyi Biotec autoMACS® Pro Separator | 2 Columns | ≤ 6 Separations |
Technical Details of Miltenyi Biotec autoMACS® Pro Separator.
Multiplex
FlowKon furthermore has a multiplex array system which affords researcher to detect and quantify intra- and extracellular cytokines, chemokines or phosphorylated proteins.
Instrument | Laser | Parameter |
|---|---|---|
| BIO-RAD Bio-Plex® 200 | 2 Lasers | ≤ 100 Analytes |
Technical details of BIO-RAD Bio-Plex® 200.