* corresponding author


Marie-Kristin Nagel, Kamila Kalinowska, Karin Vogel, Gregory Reynolds, Zhixiang Wu, Franziska Anzenberger, Mie Ichikawa, Chie Tsutsumi, Masa H. Sato, Bernhard Kuster, Sebastian Y. Bednarek, Erika Isono*

Proc Natl Acad Sci U S Aaccepted


Erika Isonoand Kamila Kalinowska

ESCRT-dependent degradation of ubiquitylated plasma membrane proteins in plants

Current Opinion in Plant Biology, accepted


Katrin Franz-Oberdorf, Andreas Langer, Ralf Strasser, Erika Isono, Quirin L. Ranftl, Christian Wunschel, Wilfried Schwab

Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP

Proteins. 2017 Jun 28. doi: 10.1002/prot.25343. [Epub ahead of print]



Kamila Kalinowska, Marie-Kristin Nagel, and Erika Isono*

Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes

Methods Mol Biol., 2016;1450:35-44.


Daniel Klionsky et al. 

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) 

Autophagy, 2016 Jan 2;12(1):1-222.



Kamila Kalinowska, Marie-Kristin Nagel, Kaija Goodman, Laura Cuyas, Franziska Anzenberger, Pascal Braun, Vicente Rubio, Marisa Otegui, and Erika Isono* 

Arabidopsis ALIX is important for recruiting the deubiquitinating enzyme AMSH to endosomes.

Proc Natl Acad Sci U S A, 112(40):E5543-51


Anna Małolepszy, Dorian Fabian Urbański, Euan K. James, Niels Sandal, Erika Isono, Jens Stougaard, and Stig Uggerhøj Andersen

The de-ubiquitinating enzyme AMSH1 is required for nodule infection and organogenesis in Lotus japonicus  

Plant Journal, 83(4):719-31


Cornelia Kolb, Marie-Kristin Nagel, Kamila Kalinowska, Jörg Hagmann, Mie Ichikawa, Franziska Anzenberger, Angela Alkofer, Masa H. Sato, Pascal Braun, and Erika Isono* 

FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis thaliana.

Plant Physiology, 167(4):1361-73



Mamoona Khan, Wilfried Rozhon, Tingting Chen, Marina Eremina, Simon Josef Unterholzner, Bernhardt Wurzinger, Andreas Bachmair, Markus Teige, Tobias Sieberer, Erika Isono and Brigitte Poppenberger 

Interplay between phosphorylation and SUMOylation events determines CESTA protein fate in brassinosteroid signaling.

Nature Communications, 5:4687.


Marie Barberon, Guillaume Dubeaux, Cornelia Kolb, Erika Isono, Enric Zelazny, and Grégory Vert 

Polarization of IRON-REGULATED TRANSPORTER 1 (IRT1) to the plant-soil interface plays crucial role in metal homeostasis. 

Proc Natl Acad Sci U S A, 111(22):8293-8.


Erika isono* and Marie-Kristin Nagel 

Deubiquitinating enzymes and their emerging role in plant biology.

Frontiers in Plant Science, 5:56. Review.


Kamila Kalinowska and Erika Isono* (2014)

Analysis of Global Ubiquitylation and Ubiquitin-Binding Domains Involved in Endosomal Trafficking

Methods Mol Biol (Plant Endosomes), 1209:189-202.


Timo Höwing, Caroline Höfle, Marie-Kristin Nagel, Erika Isono, Ralph Hückelhoven, and Christine Gietl

Endoplasmic reticulum KDEL-tailed cysteine endopeptidase 1 of Arabidopsis (AtCEP1) is involved in pathogen defense.

Frontiers in Plant Science, 5:58.


Anthi Katsiarimpa#, Alfonso Munõz, Kamila Kalinowska, Tomohiro Uemura, Enrique Rojo, and Erika Isono*, # 

The ESCRT-III interacting deubiquitinating enzyme AMSH3 is essential for degradation of ubiquitinated membrane proteins in Arabidopsis thaliana.  (equal contribution)

Plant Cell Phys (Special Focus Issue-Membrane Trafficking), 55(4):727-36.



Chitose Kami, Laure Allenbach, Melina Zourelidou, Karin Ljung, Frédéric Schutz, Erika Isono, Masaaki K. Watahiki, Kotaro T. Yamamoto, Claus Schwechheimer, and Christian Fankhauser 

PHYTOCHROME KINASE SUBSTRATE (PKS) proteins may control phototropism by modulating auxin-regulated gene expression.

Plant J, 77(3):393-403.


Anthi Katsiarimpa#Kamila Kalinowska#Franziska Anzenberger, Corina Weis, Maya Ostertag, Claus Schwechheimer, Frédéric Brunner, Ralph Hückelhoven, and Erika Isono

AMSH1 and the ESCRT-III subunit VPS2.1 are required for autophagic degradation in Arabidopsis thaliana. (#equal contribution)

Plant Cell, 25(6):2236-52.


Georg Hierl, Timo Höwing, Erika Isono, Friedrich Lottspeich, and Christine Gietl

Ex vivo processing for maturation of Arabidopsis KDEL-tailed cysteine endopeptidase 2 (AtCEP2) pro-enzyme and its storage in endoplasmic reticulum derived organelles.

Plant Mol Biol, 84(6):605-20.


Esther Dolze, Fatima Chigri, Timo Höwing, Georg Hierl, Erika Isono, Ute C. Vothknecht, and Chrisine Gietl 

Calmodulin-like protein AtCML3 mediates dimerization of peroxisomal processing protease AtDEG15 and contributes to normal peroxisome metabolism.

Plant Mol Biol, 83(6):607-624.


Corina Weis, Sebastian Pfeilmeier, Erich Glawischnig, Erika Isono, Fiona Pachl, Hannes Hahne, Bernhard Kuster, Ruth Eichmann and Ralph Hückelhoven

Co-immunoprecipitation-based identification of putative BAX INHIBITOR-1-interacting proteins involved in cell death regulation and plant - powdery mildew interactions.

Mol Plant Pathol, 14(8):791-802.



Benoît Derrien, Nicolas Baumberger, Mikhail Schepetilnikov, Corrado Viotti, Julia de Cillia, Véronique Ziegler-Graff, Erika Isono, Karin Schumacher, and Pascal Genschik

Selective degradation of the antiviral component ARGONAUTE1 by the autophagy pathway.

Proc Natl Acad Sci USA, 109(39):15942-6.



Anthi Katsiarimpa, Franziska Anzenberger, Nicole Schlager, Susanne Neubert, Marie-Theres Hauser, Claus Schwechheimer, and Erika Isono

The Arabidopsis deubiquitinating enzyme AMSH3 interacts with ESCRT-III subunits and regulates their localization.

Plant Cell, 23(8):3026-40.


Björn Willige, Erika Isono, René Richter, Melina Zourelidou, and Claus Schwechheimer 

Gibberellin controls PIN-FORMED abundance and is required for auxin transport-dependent growth and development in Arabidopsis thaliana

Plant Cell, 23(6): 2184-95.


Jana P. Hakenjos, René Richter, Esther M.N. Dohmann, Anthi Katsiarimpa, Erika Isono, and Claus Schwechheimer

MLN4924 is an efficient inhibitor of NEDD8 conjugation in plants. 

Plant Physiol, 156(2):527-36.



Erika Isono, Anthi Katsiarimpa, Isabel K. Müller, Franziska Anzenberger, York D. Stierhof, Niko Geldner, Joanne Chory, and Claus Schwechheimer

The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana.  

Plant Cell, 22(6):1826-37. 


Jacob Prestele, Georg Hierl, Christian Scherling, Stefan Hetkamp, Claus Schwechheimer, Erika Isono, Wolfram Weckwerth, Gerhard Wanner, and Christine Gietl 

Different functions of the C3HC4 zinc RING finger peroxins PEX10, PEX2, and PEX12 in peroxisome formation and matrix protein import. 

Proc Natl Acad Sci USA, 107(33):14915-20. 


Claus Schwechheimer and Erika Isono 

The COP9 signalosome and its role in plant development. 

Eur J Cell Biol, 89(2-3):163-8. Review.


Erika Isono and Claus Schwechheimer (2010)

Co-immunoprecipitation and protein blots.  

Methods Mol Biol, 655:377-87. 


Before 2010

Esther M.N. Dohmann, Mitchell Levesque, Erika Isono, Markus Schmid, and Claus Schwechheimer (2008)

Auxin responses in mutants of the Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC9 signalosome. 

Plant Physiol, 147:1369-79. 


Erika Isono, Kiyoshi Nishihara, Yasushi Saeki, Hideki Yashiroda, Naoko Kamata, Liying Ge, Takashi Ueda, Yoshiko Kikuchi, Keiji Tanaka, Akihiko Nakano, and Akio Toh-e (2007)

The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome.

Mol Biol Cell, 18:569-80.


Erika Isono and Akio Toh-e (2006)

The molecular mechanism of the assembly of the 26S proteasome in yeast. 

Tanpakushitsu Kakusan Koso (PNE). Review, Japanese. 


Erika Isono, Naoko Saito, Naoko Kamata, Yasushi Saeki, and Akio Toh-e (2005)

Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae. 

J Biol Chem, 280:6537-47.


Yasushi Saeki, Erika Isono, and Akio Toh-e (2005)

Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity. 

Methods Enzym, 399:215-27. 


Yasushi Saeki, Erika Isono, and Akio Toh-e (2005)

Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically-encodable tandem ubiquitin. 

Methods Enzym, 399:64-74.


Sachihiro Matsunaga, Wakana Uchida, Eduard Kejnovsky, Erika Isono, Francoise Moneger, Boris Vyskot, and Shigeyuki Kawano (2004)

Characterization of two SEPALLATA MADS-Box Genes from the dioecious plant Silene latifolia. 

Sex Plant Reprod, 17:189-93.


Yasushi Saeki, Erika Isono, Tomoko Oguchi, Masumi Shimada, Takayuki Sone, Hiroyuki Kawahara, Hideyoshi Yokosawa, and Akio Toh-e (2004)

Intracellularly inducible, ubiquitin hydrolase-insensitive tandem ubiquitins inhibit the 26S proteasome activity and cell division. 

Genes Genet Syst, 79:77-86. 


Erika Isono, Yasushi Saeki, Hideyoshi Yokosawa, and Akio Toh-e (2004)

Rpn7 Is required for the structural integrity of the 26S proteasome of Saccharomyces cerevisiae

J Biol Chem, 279:27168-76.


Sachihiro Matsunaga, Erika Isono, Eduard Kejnovsky, Boris Vyskot, Jaroslav Dolezel, Shigeyuki Kawano, and Deborah Charlesworth (2003)

Duplicative transfer of a MADS box gene to a plant Y chromosomome. 

Mol Biol Evol, 20:1062-9.