Peer-reviewed manuscripts

97. (preprint) Sinn M, Riede L, Fleming JR, Funck D, Lutz H, Bachmann A, Mayans O, Hartig JS. (2024). Metformin hydrolase is a recently evolved, nickel-dependent, heteromeric ureohydrolase. Research Square:

96. Mao Z, Fleming JR, Mayans O, Frey J, Schleheck D, Schink B, Müller N. (2023). AMP-dependent phosphite dehydrogenase, a phosphorylating enzyme in dissimilatory phosphite oxidation. PNAS. 120(45):e2309743120. doi: 10.1073/pnas.2309743120.

95. Lambrecht R, Delgado ME, Gloe V, Schuetz K, Plazzo AP, Franke B, Phan TS, Fleming J, Mayans O, Brunner T. (2023). Liver Receptor Homolog-1 (LRH-1/NR5A2) orchestrates hepatic inflammation and TNF-induced cell death. Cell Reports. 42(12):113513. doi: 10.1016/j.celrep.2023.113513.

94. Stehle J, Fleming JR, Bauer PM, Mayans O, Drescher M. (2023) Titin UN2A Acts as a Stable, Non-Polymorphic Scaffold in its Binding to CARP. ChemBiochem. 24(19):e202300408. doi: 10.1002/cbic.202300408.

93. Fleming JR, Hauth F, Hartig JS, Mayans O. (2023). Crystal structure of a GCN5-related N-acetyltransferase from Lactobacillus curiae. Acta Crystallogr F Struct Biol Commun. 79:217-223. doi: 10.1107/S2053230X2300571X.

92. Fleming JR, Müller I, Zacharchenko T, Diederichs K, Mayans O. (2023). Molecular insights into titin’s A-band. J Muscle Res & Cell Mot 44, 255–270. doi: 10.1007/s10974-023-09649-1.

91. Zacharchenko T, Dorendorf T, Locker N, van Dijk E, Katzemich A, Diederichs K, Bullard B, Mayans O. (2023). PK1 from Drosophila obscurin is an inactive pseudokinase with scaffolding properties. Open Biology. 13(4):220350. doi: 10.1098/rsob.220350.

90. Stergiou C, Williams R, Fleming JR, Zouvelou V, Ninou E, Andreetta F, Rinaldi E, Simoncini O, Mantegazza R, Bogomolovas J, Tzartos J, Labeit S, Mayans O*, Tzartos S*. (2023). Immunological and Structural Characterization of the Titin MIR Locus; Identification of the Middle MIR Domain, I110, as the Main Target of Titin Antibodies in Myasthenia Gravis. Biomedicines. 11(2), 449; doi: 10.3390/biomedicines11020449

89.  Sinn M , Stanoppi M , Hauth F, Fleming JR, Funck D, Mayans O, and Hartig JS. (2022). Guanidinoacid hydrolase activity of the human enzyme annotated as agmatinase. Scientific Rep. 12, 22088. doi: 10.1038/s41598-022-26655-4

88. Funck D, Sinn M, Fleming JR, Stanoppi M, Dietrich J, Mayans O, and Hartig JS (2022) Discovery of a Ni2+-dependent guanidine hydrolase in bacteria. Nature 603(7901):515-521. doi: 10.1038/s41586-022-04490-x

87. Su K, Mayans O, Diederichs K, Fleming JR, (2022) PaSiMap: Pairwise sequence similarity mapping with PaSiMap: reclassification of immunoglobulin domains from titin as case study. Computational and Structural Biotechnology Journal. 20:5409-5419. doi: 10.1016/j.csbj.2022.09.034.

86. Bogomolovas J, Gravenhorst P, Mayans O. (2022). Production and Analysis of Titin Kinase: exploiting active/inactive kinase homologs in pseudokinase validation. Meth. Enzymol. 667:147-181. doi; 10.1016/bs.mie.2022.03.028.

85. Bogomolovas J, Fleming JR, Franke B, Manso B, Simon B, Gasch A, Konzer A, Markovic M, Brunner T, Knöll R, Chen J, Labeit S, Scheffner M, Peter C, Mayans O (2021) Titin kinase ubiquitination aligns turn-over pathways with mechanical signals in the muscle sarcomere. EMBO Reports e48018 doi: 10.15252/embr.201948018

84. Porto D, Matsunaga Y, Franke B, Williams RM, Qadota H, Mayans O, Benian GM, Lu H. (2021). Conformational changes in twitchin kinase in vivo revealed by FRET imaging of freely moving C. elegans. eLIFE 2021.03.01.433414; doi: 10.1101/2021.03.01.433414.

83. Stronczek C, Lange S, Bullard B, Wolniak S, Börgeson E, Mayans O, Fleming JR. (2021) The N2A region of titin has a unique structural configuration. Journal of General Physiology. 153(7) e202012766 doi: 10.1085/jgp.202012766

82. Zhou T, Fleming JR, Lange S, Hessel A, Bogomolovas J, Stronczek C, Grundei D, Ghassemian M, Biju A, Börgeson E, Bullard B, Linke W, Chen J, Kovermann M, Mayans O. (2021). Molecular characterisation of titin N2A and its binding of CARP reveals a titin/actin cross-linking mechanism. J Mol Biol. 433(9):166901. doi: 10.1016/j.jmb.2021.166901

81. Mossanen-Parsi A, Parisi D, Browne-Marke N, Bharudin I, Connell SR, Mayans O, Fucini P, Morozov IY, Caddick MX. (2021). Histone mRNA is subject to 3’ uridylation and re-adenylation in Aspergillus nidulans. Molecular Microbiology. 115(2):238-254. doi: 10.1111/mmi.14613.

80. Franke B, Veses-Garcia M, Diederichs K, Allison A, Rigden DJ, Mayans O. (2020). Structural annotation of the conserved carbohydrate esterase vb_24B_21 from Shiga toxin-encoding bacteriophage Φ24B. J Struct Biol. 212(1):107596. doi: 10.1016/j.jsb.2020.107596.

79. Nesterenko Y, Dolde X, Leist M, Mayans (2020) Strategy to replace animal derived ECM components by a modular and highly defined matrix. ALTEX 37(3):482-489. doi: 10.14573/altex.2003181.

78. Fleming JR, Rigden DJ, Mayans O (2020) The importance of chain context in assessing small nucleotide variants in titin: in silico case study of the I10-I11 tandem and its arrhythmic right ventricular cardiomyopathy linked position T2580. J Biol Struct & Dyn. 22:1-11. doi: 10.1080/07391102.2020.1768148.

77. Lanzicher T, Zhou T, Saripalli C, Keschrumrus V, Smith Iii JE, Mayans O, Sbaizero O, Granzier H. (2020) Single-Molecule Force Spectroscopy on the N2A Element of Titin: Effects of Phosphorylation and CARP. Front Physiol. 11:173. doi: 10.3389/fphys.2020.00173.

76. Welte H, Zhou T, Mihajlenko X, Mayans O, Kovermann M. (2020) What does fluorine do to a protein? Thermodynamic, and highly-resolved structural insights into fluorine-labelled variants of the cold shock protein. Sci Rep. 10(1):2640. doi: 10.1038/s41598-020-59446-w.

75. Nesterenko Y, Hill CJ, Fleming JR, Murray P, Mayans O. (2019) The ZT Biopolymer: A Self-Assembling Protein Scaffold for Stem Cell Applications. Int J Mol Sci. 20(17). pii: E4299. doi: 10.3390/ijms20174299.

74. Stevens M, Franke B, Skorupka KA, Cafiso DS, Pornillos O, Mayans O, Norman DG. (2019) Exploration of the TRIM Fold of MuRF1 Using EPR Reveals a Canonical Antiparallel Structure and Extended COS-Box. J Mol Biol. 431(15):2900-2909. doi: 10.1016/j.jmb.2019.05.025.

73. Hill CJ, Fleming JR, Mousavinejad M, Nicholson R, Tzokov SB, Bullough PA, Bogomolovas J, Morgan MR, Mayans O[CA], Murray P[CA]. (2019) Self-Assembling Proteins as High-Performance Substrates for Embryonic Stem Cell Self-Renewal. Adv Mater. 31(17):e1807521. doi: 10.1002/adma.201807521.

72. Adams M, Fleming JR, Riehle E, Zhou T, Zacharchenko T, Markovic M, Mayans O. (2919) Scalable, Non-denaturing Purification of Phosphoproteins Using Ga(3+)-IMAC: N2A and M1M2 Titin Components as Study case. Protein J. 38(2):181-189. doi: 10.1007/s10930-019-09815-w.

71. Knorr S, Sinn M, Galetskiy D, Williams RM, Wang C, Müller N, Mayans O, Schleheck D, Hartig JS. (2018) Widespread bacterial lysine degradation proceeding via glutarate and L-2-hydroxyglutarate. Nat Commun.9(1):5071. doi: 10.1038/s41467-018-07563-6.

70. Fleming JR, Schupfner M, Busch F, Baslé A, Ehrmann A, Sterner R, Mayans O. (2018) Evolutionary Morphing of Tryptophan Synthase: Functional Mechanisms for the Enzymatic Channeling of Indole. J Mol Biol. 430(24):5066-5079. doi: 10.1016/j.jmb.2018.10.013.

69. Rigden DJ, Thomas JMH, Simkovic F, Simpkin A, Winn MD, Mayans O, Keegan RM. (2018) Ensembles generated from crystal structures of single distant homologues solve challenging molecular-replacement cases in AMPLE. Acta Crystallogr D Struct Biol. 74(Pt 3):183-193. doi: 10.1107/S2059798318002310.

68. Williams RM, Franke B, Wilkinson M, Fleming JR, Rigden DJ, Benian GM, Eyers PA, Mayans O. (2018) Autophosphorylation Is a Mechanism of Inhibition in Twitchin Kinase. J Mol Biol. 430(6):793-805. doi: 10.1016/j.jmb.2018.01.020.

67. Thomas JMH, Simkovic F, Keegan R, Mayans O, Zhang C, Zhang Y, Rigden DJ. (2017) Approaches to ab initio molecular replacement of α-helical transmembrane proteins. Acta Crystallogr D Struct Biol. 73(Pt 12):985-996. doi: 10.1107/S2059798317016436.

66. Matsunaga Y, Hwang H, Franke B, Williams R, Penley M, Qadota H, Yi H, Morran LT, Lu H, Mayans O, Benian GM. (2017) Twitchin kinase inhibits muscle activity. Mol Biol Cell. 28(12):1591-1600. doi: 10.1091/mbc.E16-10-0707.

65. Zacharchenko T, Barsukov I, Rigden DJ, Bennett D, Mayans O. (2016). Biophysical analysis of the N-terminal domain from the human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS suggests an extended transcription factor TFIIS-like fold. Protein J. 35(5):340-345. doi: 10.1007/s10930-016-9677-7.

64. Zhou T, Fleming J, Franke B, Bogomolovas J, Barsukov I, Rigden DJ, Labeit S, Mayans O. (2016). CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex. FEBS Letters. 590(18):3098-110. doi: 10.1002/1873-3468.12362.

63. Bogomolovas J, Fleming J, Anderson BR, Williams R, Lange S, Simon B, Khan MM, Rudolf R, Franke B, Bullard B, Rigden DJ, Granzier H, Labeit S, Mayans O. (2016). Exploration of pathomechanisms triggered by a single-nucleotide polymorphism in titin’s I-band: the cardiomyopathy-linked mutation T2580I. Open Biology. Sep;6(9). pii: 160114. doi: 10.1098/rsob.160114.

62. Simkovic F, Thomas J, Keegan RM, Winn MD, Mayans O, Rigden DJ. (2016). Residue contacts predicted by evolutionary covariance extend the application of ab initio Molecular Replacement to larger and more challenging protein folds. IUCr J. 3:259-270. doi: 10.1107/S2052252516008113.

61. Qadota H, Mayans O, Matsunaga Y, McMurry JL, Wilson KJ, Kwon GE, Stanford R, Deehan K, Tinley TL, Ngwa VN, Benian GM. (2016). The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in C. elegans muscle. Mol Biol Cell. 27(10):1606-1620. doi: 10.1091/mbc.E15-09-0675.

60. Zacharchenkov T, von Castelmur E, Rigden DJ, Mayans O. (2015). Structural advances on titin: towards the atomic understanding of multi-domain functions in myofilament mechanics and scaffolding. Biochem Soc Trans. 43(5):850-855. doi: 10.1042/BST20150084.

59. Thomas JMH, Keegan RM, Bibby J, Winn MD, Mayans O, Rigden DJ. (2015). Routine phasing of crystal structures of coiled-coil proteins with AMPLE. IUCr J. 2(2):198-206. doi: 10.1107/S2052252515002080.

Highlighted in: Commentary by Zbigniew Dauter “Solving coiled-coil protein structures” (2015). IUCr J.2:164-165. doi: 10.1107/S2052252515003486.

58. Pandalaneni S, Karuppiah V, Saleem M, Haynes LP, Burgoyne RD, Mayans O, Derrick JP, Lian L-Y. (2015). Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions. J Biol Chem. 290(30):18744-56. doi: 10.1074/jbc.M114.627059.

57. Schneider D, Kaiser W, Stutz C, Holinski A, Mayans O, Babinger P. (2015). YbiB from E. coli, the defining member of the novel TrpD2 family of prokaryotic DNA binding proteins. J Biol Chem. 290(32):19527-39. doi: 10.1074/jbc.M114.620575.

56. Benian GM, Mayans O. (2015). “Titin and obscurin: Giants holding hands and discovery of a new Ig subset”. J Mol Biol. 427(4):707-714. doi: 10.1016/j.jmb.2014.12.017.

55. Keegan RM, Bibby J, Thomas J, Xu D, Zhang Y, Mayans O, Winn MD, Rigden DJ. (2015). Exploring the speed and performance of molecular replacement with AMPLE using QUARK ab initio protein models. Acta Cryst D71. 71(2):338-343. doi: 10.1107/S1399004714025784.

54. Mitchell SN, Rigden DJ, Dowd AJ, Lu F, Wilding CS, Weetman D, Dadzie S, Jenkins AM, Regna K, Boko P, Djogbenou L, Muskavitch MAT, Ranson H, Paine MJI, Mayans O[CA], Donnelly MJ[CA]. (2014). Metabolic and Target-Site Mechanisms Combine to Confer Strong DDT Resistance in Anopheles gambiae. PloS One.9(3):e92662. doi: 10.1371/journal.pone.0092662.

53. Franke B, Gasch A, Rodriguez D, Chami M, Khan MM, Rudolf R, Bibby J, Hanashima A, Bogomolovas J, von Castelmur E, Rigden DJ, Uson I, Labeit S, Mayans O. (2014). Molecular basis for the fold organization and sarcomeric targeting of the muscle atrogin MuRF1. Open Biology. 4(3):130172. doi: 10.1098/rsob.130172.

52. Bogomolovas J, Gash A, Simkovic F, Rigden DJ, Labeit S, Mayans O. (2014). Titin kinase is an inactive pseudokinase scaffold that supports MuRF1 recruitment to the sarcomeric M-line. Open Biology. 4(5):140041. doi: 10.1098/rsob.140041.

51. Busch F, Rajendran C, Mayans O, Löffler P, Merkl R, Sterner R. (2014). TrpB2 enzymes are O-phospho-L-serine dependent tryptophan synthases. Biochemistry. 53(38):6078-6083. doi: 10.1021/bi500977y.

50. Nayeem N, Mayans O, Green T. (2013). Correlating Efficacy and Desensitization with GluK2 Ligand-Binding Domain Movements. Open Biology. 3(5):130051. doi: 10.1098/rsob.130051.

49. Mayans O[CA], Benian G, Simkovic, F, Rigden D. (2013). Mechanistic and functional diversity in the mechanosensory kinases of the titin-like family. Biochem Soc Trans. 41(4):1066-71. doi: 10.1042/BST20130085.

48. Bibby J, Keegan RM, Mayans O, Winn MD, Rigden DJ. (2013). Application of the AMPLE cluster-and-truncate approach to NMR structures for molecular replacement. Acta Cryst D. 69:2194-201. doi: 10.1107/S0907444913018453.

47. Bruning M, Barsukov I, Franke B, Barbieri S, Volk M, Leopoldseder S, Ucurum Z, Mayans O. (2012). The intracellular Ig fold: a robust protein scaffold for the engineering of molecular recognition. Prot Eng Design & Selec. 25(5):205-12. doi: 10.1093/protein/gzs007.

46. Mayans O[CA], Labeit S. (2012). “MuRFs: specialized members of the TRIM/RBCC family with roles in the regulation of the trophic state of muscle and its metabolism.” Adv Exp Med Biol. 770:119-129. doi: 10.1007/978-1-4614-5398-7_9. (Revised reprint of book chapter)

45. Von Castelmur E, Strümpfer J, Franke B, Bogomolovas J, Barbieri S, Qadota H, Konarev PV, Svergun DI, Labeit S, Benian GM, Schulten K, Mayans O. (2012) Identification of an N-terminal inhibitory extension as the primary mechanosensory regulator of twitchin kinase. Proc Nat Acad Sci. 109(34):13608-13. doi: 10.1073/pnas.1200697109.

44. Bibby J, Keegan R, Mayans O, Wynn M, Rigden D. (2012). AMPLE: a cluster-and-truncate approach to solve crystal structures of small proteins using rapidly computed ab initio models. Acta Cryst D. 68(12):1622-1631. doi: 0.1107/S0907444912039194.

43. Nayeem N, Mayans O, Green T. (2011). Conformational flexibility of the ligand-binding domain dimer in kainate receptor gating and desensitization. J Neurosciences. 31(8):2916-2924. doi: 10.1523/JNEUROSCI.4771-10.2011.

42. Chami M, Franke B, Mayans O, Kreplak L. (2011) Assembly of a protein ‘brush’ by end-grafting titin fragments to liposomes. J Biosc & Bioeng. 112(2):178-179. doi: 10.1016/j.jbiosc.2011.04.014.

41. Lee EH, Hsin J, von Castelmur E, Mayans O, Schulten K. (2010). Tertiary and Secondary Structure Elasticity of a Six-Ig Titin Chain. Biophysics J. 98(6):1085-1095. doi: 10.1016/j.bpj.2009.12.4192.

40. Bucher RM, Svergun DI, Muhle-Goll C, Mayans O. (2010). The structure of the FnIII tandem A77-A78 points to a periodically conserved architecture in the myosin-binding region of titin. J Mol Biol. 401(5):843-853. doi: 10.1016/j.jmb.2010.06.011.

39. Bruning M, Kreplak L, Leopoldseder S, Müller SA, Ringler P, Duchesne L, Fernig DG, Engel A, Ucurum-Fotiadis Z, Mayans O. (2010). Bipartite design of a self-assembled protein copolymer with nanopatterned peptide display capabilities. Nano Letters. 10(11):4533-4537. doi: 10.1021/nl1024886.

38. Schlee S, Deuss M, Bruning M, Ivens A, Schwab T, Hellmann N, Mayans O, Sterner R. (2009) Activation of anthranilate phosphoribosyl transferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release. Biochemistry. 48(23):5199-5209. doi: 10.1021/bi802335s.

37. Schwab T, Skegro D, Mayans O, Sterner R. (2008). A rationally designed monomeric variant of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus is as active as the dimeric wild-type enzyme but less thermostable. J Mol Biol. 376(2): 506-516. doi: 10.1016/j.jmb.2007.11.078.

36. von Castelmur E, Marino M, Svergun DI, Kreplak L, Ucurum-Fotiadis Z, Konarev PV, Urthumzshev A, Labeit D, Labeit S, Mayans O. (2008). A regular pattern of Ig super-motifs defines segmental flexibility as the elastic mechanism of the titin chain. Proc Nat Acad Sci. 105(4):1186-1191. doi: 10.1073/pnas.0707163105.

Structure highlighted in: Protein Data Bank - Titin: Molecule of the month (May, 2015)

35. Urzhumtsev A, von Castelmur E, Mayans O. (2008). Ultra-low resolution ab initio phasing of filamentous proteins: crystals from a six-Ig fragment of titin as a case study. Acta Cryst D. 64:478-486. doi: 10.1107/S0907444908003442.

34. Mrosek M, Meier S, Ucurum-Fotiadis Z, von Castelmur E, Hedbom E, Lustig A, Grzesiek S, Labeit D, Labeit S, Mayans O. (2008). Structural analysis of the B-box 2 from MuRF1: Identification of a novel self-association pattern in a RING-like fold. Biochemistry. 47(40):10722-10730. doi: 10.1021/bi800733z.

33. Snyers L, Vlcek S, Dechat T, Skegro D, Korbei B, Gajewski A, Mayans O, Schöfer C, Foisner R. (2007). Lamina-associated polypeptide 2-a forms homo-trimers via its C-terminus and oligomerization is unaffected by a disease-causing mutation. J Biol Chem. 282(9):6308-6315. doi: 10.1074/jbc.M605782200.

32. Mrosek M, Labeit D, Witt S, Heerklotz H, von Castelmur E, Labeit S, Mayans O. (2007). Molecular determinants for the recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin. FASEB J. 21(7):1383-1392. doi: 10.1096/fj.06-7644com.

31. Lee EH, Hsin J, Mayans O, Schulten K. (2007). Secondary and tertiary structure elasticity of titin Z1Z2 and a titin chain model. Biophys J. 93(5):1719-1735. doi: 10.1529/biophysj.107.105528.

30. Zou P, Pinotsis N, Lange S, Song YH, Popov A, Mavridis I, Mayans O, Gautel M, Wilmanns M. (2006). Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk. Nature. 439:229-233. doi: 10.1038/nature04343.

29. Marino M, Deuss M, Svergun DI, Konarev PV, Sterner R, Mayans O. (2006). Structural and mutational analysis of substrate complexation by anthranilate phosphoribosyl-transferase from Sulfolobus solfataricus. J Biol Chem. 281(30):21410-21421. doi: 10.1074/jbc.M601403200.

28. Garcia P, Ucurum Z, Bucher R, Svergun DI, Huber T, Lustig A, Konarev PV, Marino M, Mayans O. (2006). Molecular insights into the self-assembly mechanism of dystrophia myotonica kinase. FASEB J. 20:1142-1151. doi: 10.1096/fj.05-5262com.

27. Marino M, Zou P, Svergun DI, Garcia P, Edlich C, Simon B, Wilmanns M, Muhle-Goll C, Mayans O. (2006). The Ig-doublet Z1Z2: a model system for the hybrid analysis of conformational dynamics in poly-Ig tandems from titin. Structure 14(9):1437-1447. doi: 10.1016/j.str.2006.07.009.

26. Marino M, Svergun DI, Kreplak L, Konarev PV, Maco B, Labeit D, Mayans O. (2005). Poly-Ig tandems from I-band titin share extended domain arrangements irrespective of the distinct features of their modular constituents. J Muscle Res & Cell Mot. 26:355-365. doi: 10.1007/s10974-005-9017-6.

25. Garcia P, Marino M, Mayans O. (2004). Crystallization and preliminary X-ray analysis of the coiled-coil domain of dystrophia myotonica kinase. Acta Cryst D. 60:2336-2339. doi: 10.1107/S0907444904026873.

24. Linden A, Mayans O, Meyer-Klaucke W, Antranikian G, Wilmanns M. (2003). Differential regulation of a hyperthermophilic alpha-amylase with a novel (Ca,Zn) two-metal center by zinc. J Biol Chem. 278:9875-9884. doi: 10.1074/jbc.M211339200.

23. Ivens A, Mayans O, Szadkowski H, Jürgens C, Wilmanns M, Kasper K. (2002). Stabilization of a (ba)8-barrel protein by an engineered disulfide bond. Eur J Biochem. 269(4):1145-1153. doi: 10.1046/j.1432-1033.2002.02745.x.

22. Mayans O, Ivens A, Nissen LJ, Kirschner K, Wilmanns M. (2002). Structural analysis of two enzymes catalysing reverse metabolic reactions implies common ancestry. EMBO J. 21(13):3245-3254. doi: 10.1093/emboj/cdf298.

21. Douangamath A, Filipp FV, Klein ATJ, Barnett P, Zou P, Voorn-Brouwer T, Vega MC, Mayans OM, Sattler M, Distel B, Wilmanns M. (2002). Topography for independent binding of a-helical and PPII-helical ligands to a peroxisomal SH3 domain. Mol Cell. 10:1007-1017. doi: 10.1016/s1097-2765(02)00749-9.

20. Jenkins J, Mayans O, Smith D, Worboys K, Pickersgill RW. (2001). Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site. J Mol Biol. 305:951-960. doi: 10.1006/jmbi.2000.4324.

19. Ivens A, Mayans O, Szadkowski H, Wilmanns M, Kirschner K. (2001). Purification, characterization and crystallization of recombinant anthranilate phosphoribosyltransferase from Sulfolobus solfataricus". Eur J Biochem. 268(8):2246-2252. doi: 10.1046/j.1432-1327.2001.02102.x.

18. Mayans O, Wuerges J, Canela S, Gautel M, Wilmanns M. (2001). Structural evidence for a possible role of reversible disulphide bridge formation in the elasticity of the muscle protein titin. Structure. 9(4):331-340. doi: 10.1016/s0969-2126(01)00591-3.

17. Wilmann M, Gautel M, Mayans O. (2000). Activation of calcium/calmodulin regulated kinases. Cell Mol Biol (Noisy-le-grand). 46(5):883-894.

16. Mayans O, Wilmanns M. (1999). X-ray analysis of protein crystals with thin-plate morphology. J Synchrotron Radiation. 6:1016-1020. doi: 10.1107/S0909049599004616.

15. Mayans O, van der Ven PFM, Wilm M, Mues A, Young P, Fuerst DO, Wilmanns M, Gautel M. (1998). Structural basis for activation of the titin kinase domain during myofibrillogenesis. Nature. 395:863-869. doi: 10.1038/27603.

Highlighted in: News and Views - Commentary by Means AR. “The clash in titin” (1998) 395(6705):846-847. doi: 10.1038/27557.

14. Shevchik V, Scott M, Mayans O, Jenkins J. (1998). Crystallization and preliminary X-ray analysis of a member of a new family of pectate lyases, PelL from Erwinia chrysanthemi. Acta Cryst D. 54:419-422. doi: 10.1107/s0907444997012043.

13. Jenkins J, Mayans O, Pickersgill R. (1998). Structure and evolution of parallel b-helix proteins. J Struct Biol. 122:236-246. doi: 10.1006/jsbi.1998.3985.

12. Pickersgill R, Harris G, Lo Leggio L, Mayans O, Jenkins J. (1998). Superfamilies: The 4/7 superfamily of ba-barrel glycosidases and the right-handed parallel b-helix superfamily. Biochem Soc Trans. 26:190-198. doi: 10.1042/bst0260190.

11. Mayans O, Scott M, Connerton I, Gravesen T, Benen J, Visser J, Pickersgill R, Jenkins J. (1997). Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate binding clefts of pectin and pectate lyases. Structure, 5:677-690. doi: 10.1016/s0969-2126(97)00222-0.

10. Boehm MK, Mayans MO, Thornton JD, Begent RH, Keep PA, Perkins SJ. (1996). Extended glycoprotein structure of the 7 domains in human carcinoembryonic antigen by X-ray and neutron solution scattering and an automated curve-fitting procedure - implications for cellular adhesion. J Mol Biol, 259:718-736. doi: 10.1006/jmbi.1996.0353.

9. Jenkins J, Scott M, Mayans O, Pickersgill R, Harris G, Connerton I, Gravesen T. (1996). "Crystallization and preliminary X-ray analysis of pectin lyase A from Aspergillus niger". Acta Cryst D. 52:402-404. doi: 10.1107/S0907444995012698.

8. Meyer DF, Mayans MO, Groot PH, Suckling KE, Bruckdorfer KR, Perkins SJ. (1995). Time-course studies by neutron solution scattering and biochemical assays of the aggregation of human Low Density Lipoprotein during Cu2+-induced oxidation. Biochem J. 310:417-426. doi: 10.1042/bj3100417.

7. Mayans MO, Coadwell WJ, Symons DB, Perkins SJ. (1995). Demonstration by pulsed-neutron scattering that the arrangement of the Fab and Fc fragments in the overall structures of bovine IgG1 and IgG2 in solution is similar. Biochem J. 311:283-291. doi: 10.1042/bj3110283.


Book chapters, Newsletters, Editorials

6. (Reprint): Nesterenko Y, Hill CJ, Fleming JR, Murray P, Mayans O. (2020) “The ZT Biopolymer: A Self-Assembling Protein Scaffold for Stem Cell Applications.” In Designer Biopolymers: Self-Assembling Proteins and Nucleic Acids. Edited by Ayae Sugawara-Narutaki and Yukiko Kamiya. Published by MDPI. ISBN 978-3-03936-370-4 (Hbk); ISBN 978-3-03936-371-1; doi: 10.3390/ijms20174299.

5. Matusovsky OS, Mayans O, Szczesna-Cordary D. (2015). “Molecular mechanism of muscle contraction: new perspectives and ideas.” Biomed Res Int. 201510.1155.

4. Bibby J, Keegan R, Rigden D, Wynn M, Mayans O. (2012). AMPLE – using ab initio modelling to tackle difficult molecular replacement cases. CCP4 NewsLetter Number 48: August 2012

3. Mayans O, Labeit S. (2009). “MuRFs: specialized members of the TRIM/RBCC family with roles in the regulation of the trophic state of muscle and its metabolism”. Book chapter in TRIM/RBCC Proteins. Landes Biosciences. Edited by Germana Meroni. (Epub: doi: 10.1007/978-1-4614-5398-7_9.

2. Mayans O, Jenkins J, Smith D, Worboys K. (1998). "Pectin modifying enzymes and designer pectins". In Protein Engineering in Industrial Biotechnology. Ed. Alberghina & L. Harwood. Academic Press. Amtersdam.

1. Read CM, Cary PD, Crane-Robinson C, Driscoll PD, Mayans-Carrillo OM, Norman DG. (1995)."The structure of the HMG Box and its Interaction with DNA". Book chapter in Nucleic Acids and Molecular Biology Vol. 9, edited by Eckstein, F. & Lilley, D.M. Published by Springer-Verlag Berlin and Heidelberg GmbH & Co. KG. pp. 222-250.

Protein Data Bank Entries

  1. 1IDJ - Pectin Lyase A (1997) (doi: 10.2210/pdb1IDJ/pdb)
  2. 1IDK - Pectin Lyase A (1997) (doi: 10.2210/pdb1IDK/pdb)
  3. 1TKI - Autoinhibited Serine Kinase Domain Of The Giant Muscle Protein Titin (1999) (doi: 10.2210/pdb1TKI/pdb)
  4. 1QJV - Pectin Methylesterase PemA from Erwinia chrysanthemi (2000) (doi: 10.2210/pdb1QJV/pdb)
  5. 1G1C - I1 Domain from Titin (2001) (doi: 10.2210/pdb1G1C/pdb)
  6. 1JCM - TrpC Stability Mutant Containing an Engineered Disulphide Bridge and in Complex with a Cdrp-Related Substrate (2002) (doi: 10.2210/pdb1JCM/pdb)
  7. 1O17 - Anthranilate Phosphoribosyl-Transferase (TrpD) (2002) (doi: 10.2210/pdb1O17/pdb)
  8. 1JQQ - Crystal Structure of Pex13p(301-386) SH3 domain (2002) (doi: 10.2210/pdb1JQQ/pdb)
  9. 1N5Z - Complex Structure of Pex13p SH3 domain with a peptide of Pex14p (2002) (doi: 10.2210/pdb1N5Z/pdb)
  10. 1GXB - Anthranilate Phosphoribosyltransferase in Complex with Pyrophosphate and Magnesium (2003) (doi: 10.2210/pdb1GXB/pdb)
  11. 1MWO - Crystal Structure Analysis of the Hyperthermostable Pyrocoocus woesei alpha-amylase (2003) (doi: 10.2210/pdb1MWO/pdb)
  12. 1MXD - Structure of a (Ca,Zn)-dependent alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei (2003) (doi: 10.2210/pdb1MXD/pdb)
  13. 1MXG - Crystal Structure of a (Ca,Zn)-dependent alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei in complex with acarbose (2003) (doi: 10.2210/pdb1MXG/pdb)
  14. 1WT6 - Coiled-coil domain of DMPK (2005) (doi: 10.2210/pdb1WT6/pdb)
  15. 1YA5 - Crystal structure of the titin domains Z1Z2 in complex with telethonin (2005) (doi: 10.2210/pdb1YA5/pdb)
  16. 1ZYK - Anthranilate Phosphoribosyltransferase in complex with PRPP, anthranilate and magnesium (2006) (doi: 10.2210/pdb1ZYK/pdb)
  17. 1ZXY - Anthranilate Phosphoribosyltransferase from Sulfolobus solfataricus in complex with PRPP and Magnesium (2006) (doi: 10.2210/pdb1ZXY/pdb)
  18. 2GVQ - Anthranilate phosphoribosyl-transferase (TRPD) from S. solfataricus in complex with anthranilate (2006) (doi: 10.2210/pdb2GVQ/pdb)
  19. 2A38 - Crystal structure of the Z1Z2 N-Terminus of titin (2006) (doi: 10.2210/pdb2A38/pdb)
  20. 2NZI - Crystal structure of domains A168-A170 from titin (2007) (doi: 10.2210/pdb2NZI/pdb)
  21. 2RIK - I-band fragment I67-I69 from titin (2008) (doi: 10.2210/pdb2RIK/pdb)
  22. 2RJM - 3Ig structure of titin domains I67-I69 E-to-A mutated variant (2008) (doi: 10.2210/pdb2RJM/pdb)
  23. 3B43 - I-band fragment I65-I70 from titin (2008) (doi: 10.2210/pdb3B43/pdb)
  24. 3DDT - Crystal structure of the B2 box from MuRF1 in dimeric state (2008) (doi: 10.2210/pdb3DDT/pdb)
  25. 3GBR - Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus (2009) (doi: 10.2210/pdb3GBR/pdb)
  26. 3LPW - Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin (2010) (doi: 10.2210/pdb3LPW/pdb)
  27. 2XXR - Crystal structure of the GluK2 (GluR6) wild-type LBD dimer in complex with glutamate (2011) (doi: 10.2210/pdb2XXR/pdb)
  28. 2XXT - Crystal structure of the GluK2 (GluR6) wild-type LBD dimer in complex with kainite (2011) (doi: 10.2210/pdb2XXT/pdb)
  29. 2XXU - Crystal structure of the GluK2 (GluR6) M770K LBD dimer in complex with glutamate (2011) (doi: 10.2210/pdb2XXU/pdb)
  30. 2XXV - Crystal structure of the GluK2 (GluR6) M770K LBD dimer in complex with kainite (2011) (doi: 10.2210/pdb2XXV/pdb)
  31. 2XXW - Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with glutamate (2011) (doi: 10.2210/pdb2XXW/pdb)
  32. 2XXX - Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with glutamate (P21 21 21) (2011) (doi: 10.2210/pdb2XXX/pdb)
  33. 2XXY - Crystal structure of the GluK2 (GluR6) D776K LBD dimer in complex with kainite (2011) (doi: 10.2210/pdb2XXY/pdb)
  34. 3UTO - Twitchin kinase region from C. elegans (Fn31-NL-kin-CRD-Ig26) (2012) (doi: 10.2210/pdb3UTO/pdb)
  35. 4BDL - Crystal structure of the GluK2 K531A LBD dimer in complex with glutamate (2013) (doi: 10.2210/pdb4BDL/pdb)
  36. 4BDM - Crystal structure of the GluK2 K531A LBD dimer in complex with kainite (2013) (doi: 10.2210/pdb4BDM/pdb)
  37. 4BDN - Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with glutamate (2013) (doi: 10.2210/pdb4BDN/pdb)
  38. 4BDO - Crystal structure of the GluK2 K531A-T779G LBD dimer in complex with kainite (2013) (doi: 10.2210/pdb4BDO/pdb)
  39. 4BDQ - Crystal structure of the GluK2 R775A LBD dimer in complex with glutamate (2013) (doi: 10.2210/pdb4BDQ/pdb)
  40. 4BDR - Crystal structure of the GluK2 R775A LBD dimer in complex with kainite (2013) (doi: 10.2210/pdb4BDR/pdb)
  41. 4OAJ - Crystal structure of the complex between SAP97 PDZ2 and 5HT2A receptor peptide (2014) (doi: 10.2210/pdb4OAJ/pdb)
  42. 4GSN - Crystal Structure of GSTe2 ZAN/U variant from Anopheles gambiae (2014) (doi: 10.2210/pdb4GSN/pdb)
  43. 4M3L - Crystal Structure of the coiled coil domain of MuRF1 (2014) (doi: 10.2210/pdb4M3L/pdb)

  44. 4OV2 - Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus (2014) (doi: 10.2210/pdb4OV2/pdb)

  45. 4JNW - Bacterially expressed Titin Kinase (2014) (doi: 10.2210/pdb4JNW/pdb)

  46. 4MUO - The TrpD2 enzyme from E.coli: YbiB (2013) (doi: 10.2210/pdb4MUO/pdb)
  47. 4YRU - Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus (2015) (doi: 10.2210/pdb4YRU/pdb)
  48. 4QEG - Crystal structure of domain I10 from titin (space group P41) (2015) (doi: 10.2210/pdb4QEG/pdb)
  49. 5JDJ - Crystal structure of domain I10 from titin in space group P212121 (2016) (doi: 10.2210/pdb5JDJ/pdb)
  50. 5JOE - Crystal structure of I81 from titin (2016) (doi: 10.2210/pdb5JOE/pdb)
  51. 5JDD - Crystal structure of I9-I11 tandem from titin (P212121) (2016) (doi: 10.2210/pdb5JDD/pdb)
  52. 5JDE - Crystal structure of I9-I11 tandem from titin (P1) (2016) (doi: 10.2210/pdb5JDE/pdb)
  53. 5N2P - Sulfolobus solfataricus Tryptophan Synthase A (2018) (doi: 10.2210/pdb5N2P/pdb)
  54. 6HUL - Sulfolobus solfataricus Tryptophan Synthase AB Complex (2018) (doi: 10.2210/pdb6HUL/pdb)
  55. 6HTE - Sulfolobus solfataricus Tryptophan Synthase B2a (2018) (doi: 10.2210/pdb6HTE/pdb)
  56. 6EQN - Sulfolobus solfataricus Tryptophan Synthase B2o (2018) (doi: 10.2210/pdb6EQN/pdb)
  57. 6GPE - Crystal Structure of the CsiD Glutarate Hydroxylase (2018) (doi: 10.2210/pdb6GPE/pdb)
  58. 6HL8 - Crystal Structure of the CsiD Glutarate Hydroxylase in complex with Glutarate (2018) (doi: 10.2210/pdb6HL8/pdb)
  59. 6GPN - Crystal Structure of the CsiD Glutarate Hydroxylase in complex with N-Oxalylglycine (2019) (doi: 10.2210/pdb6GPN/pdb)
  60. 6HL9 - Crystal Structure of the CsiD Glutarate Hydroxylase in complex with Succinate (2019) (doi: 10.2210/pdb6HL9/pdb)
  61. 6FWX - Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin (2019) (doi: 10.2210/pdb6FWX/pdb)
  62. 6SDB - Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin (2019) (doi: 10.2210/pdb6SDB/pdb)
  63. 6SZZ - Crystal structure of Cold Shock Protein B (CspB) containing the modified residue 4-F-Trp (2020) (doi: 10.2210/pdb6SZZ/pdb)
  64. 6T00 - Crystal structure of Cold Shock Protein B (CSP-B) containing 4-F-Phe modified residues (2020) (doi: 10.2210/pdb6T00/pdb)
  65. 6YP6 - Structural annotation of the conserved carbohydrate esterase vb_24B_21 from Shiga toxin-encoding bacteriophage phi24B (2020) (doi: 10.2210/pdb6YP6/pdb)
  66. 7NIP  titin N2A unique sequence (UN2A) core (2021) (doi: 10.2210/pdb7NIP/pdb)
  67. 7AHS - titin-N2A Ig81-Ig83 (2021) (doi: 10.2210/pdb7AHS/pdb)
  68. 6YGN - Titinkinase and its flanking domains (2021) (doi: 10.2210/pdb6YGN/pdb)
  69. 7ESR - Crystal structure of Synechocystis sp PCC6803 guanidinium hydrolase (R32) (2021) (doi: 10.2210/pdb7ESR/pdb)

  70. 7OI1 - Crystal structure of Synechocystis sp PCC6803 guanidinium hydrolase (2021) (doi: 10.2210/pdb7OI1/pdb)

  71. 8BVO: Titin I110-I111 FnIII tandem from the MIR region (I/A5-I/A6) (2023) (doi: 10.2210/pdb8BVO/pdb)

  72. 8BW6:  Titin FnIII-domain I110 (I/A6) from the MIR region (2023) (doi: 10.2210/pdb8BW6/pdb)

  73. 8BXR: Titin FnIII-domain I109-I111 (I/A4-A/A6) from the MIR region (2023) (doi: 10.2210/pdb8BXR/pdb)

  74. 8AK2: Drosophila melanogaster UNC89/Obscurin Protein Kinase Domain 1 (apo) (2023) (doi: 10.2210/pdb8AK2/pdb)

  75. 8AK3: Drosophila melanogaster UNC89/Obscurin Protein Kinase 1 in complex with ADP  (2023) (doi: 10.2210/pdb8AK3/pdb)

  76. 8BNQ: Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin  (2023) (doi: 10.2210/pdbBNQ/pdb)

  77. 8OSP: GCN5-related N-Acetyltransferase from Lactobacillus curiae  (2023) (doi: 10.2210/pdb8OSP/pdb)


Mayans O, Kreplak L, Bruning M. (Sept 2009) “Self-assembling protein fibers” Patent Nr. GB0915746.2 / GB2473295-A. Patent Assignee: University of Basel.

Hill C, Murray P, Mayans O. “Protein-composed extracellular matrix mimetic for embryonic stem cell culture and self-renewal” Patent Nr: WO2019030524-A1/2019 (Derwent Accession Nr: 2019-15321J) Patent Assignee: University of Liverpool.