Applications

Sorting via flow cytometry serves as a starting point for further analysis of specific cell or particle populations out of a heterogeneous sample. This comprises the generation of stable cell lines by single cell sorting of transfected or transduced cells as well as enrichment of populations of cells or (biological or non-biological) particles by a bulk sort. Particles of interest can be already specified by their scatter properties passing a laser beam. However, depending on the complexity of a sample one or more fluorescence parameters are needed to define a population. The size of particles to sort should be between 1 and 20 µm. One specified population can be collected in different plates (96-well, 12-well, 6-well), two or up to four different populations can be collected in tubes (15 mL conical or 5 mL round bottom). Besides the standard application of creating cell lines (HEK293, HeLa, THP-1, SaOS2, HL-60) and enrichment of primary cells (mouse lymphocytes, thymocytes), we have already sorted E. coli, particles from C. elegans and from plants. In any case if you want to sort a standard or a new kind of sample please contact us in advance to discuss details of your experiment (flowkon@uni-konstanz.de).