Instruments at other groups

Please contact the indicated person if you want to work on the instruments mentioned in this section.

The instruments are not maintained and operated by the facility. Therefore, usage fees in form of buffer, disinfectant or other reagents have to be arranged by the user with the respective working group members or the indicated principal investigators.

   

Amnis ImageStreamX Mk II Imaging Flow Cytometer

Room U

Prof. Lutz Becks ( +49 7531 88-2828)

The ImageStream®X Mark II System is a multispectral, imaging flow cytometer that combines the visual power of microscopy with the speed, sensitivity, and phenotyping abilities of flow cytometry by digitally imaging cells directly in flow. 

The instrument is equipped with two CCD cameras, a brightfield lamp and three lasers - blue (488nm), violet (405nm) and red (642nm) - enabling 12 high-resolution images (brightfield, darkfield and up to 10 fluorescence parameters) of each cell at 20x, 40x or 60x magnification and rates up to 5,000 cells per second.

With these features the image stream enables a broad range of quantitative, statistically robust cellular analysis is ideal to investigate cell states, cell signaling, nuclear and co-localisation, internalisation, shape change and much more.

    

BD Accuri™ C6

Room P1114, Groettrup Lab

PD Dr. Michael Basler (+49 7531  88-2258)

Laser and filters

488 nm laser  

FITC, GFP, YFP, CFSE, Alexa Fluor 488

530/30

PE, PI, PE-CFS94

585/40

PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy7, PI, 7-AAD

670 LP
640 nm laser  

APC, Alexa Fluor 647                                      

 675/25

BD Accuri™ C6 Flow Cytometer Optical Filter Guide

Software

BD CSamplerTM software based on BD AccuriTM C6 software

Special equipment

CSampler compatible with

  • 24 tube rack
  • 48 well plate
  • 96 well plate: U-bottom, flat-bottom, V-bottom
  • 96 deep well plate

BD LSRFortessa™

Room L908, Brunner Lab,

Prof. Dr. Thomas Brunner (+49 7531 88-5371)

Laser and filters

405 nm laser  

Alexa Fluor 405, Pacific Blue, DAPI, Hoechst

450/50

AmCyan, CFP                                      

525/50, 505 LP

QDot 605

610/20, 600 LP
488 nm laser  

SSC 

488/10

FITC, GFP, Alexa Fluor 488                      

530/30, 505 LP

PerCP

710/50, 685 LP
 561 nm laser  

PE, DsRed, Rhodamine Red

586/15, 575 LP

PE-Texas Red, PI, mCherry                    

610/20, 600 LP

PE-Cy5

670/30, 635 LP

PerCP-Cy5.5

710/50, 685 LP

PE-Cy7

780/60, 750 LP
 633 nm laser  

APC

670/14

Alexa Fluor 680, Alexa Fluor 700               

730/45, 690 LP

APC-Cy7, APC-H7

780/60, 750 LP

BD LSRFortessaTM Cell Analyzer Bandpass Filter Guide

Software

BD FACSDivaTM

Special equipment

BDTM High Throughput Sampler (HTS) compatible with

  • 96 well plate
  • 384 well plate

COPAS FP-250

ML7, Deuerling Group,

Dr. Martin Gamerdinger (+49 7531  88-2039)

Description

The instrument is primarily used for sorting of all stages of C. elegans. It is also suitable for isolation of cell clusters like mouse adipocytes, ESC's / EB's, hepatocytes, pancreatic islets, and duct cells as well as small beads and seeds with sizes from 20 to 200 microns (diameter).