Publications

[CA] = corresponding author; & = co-author 

26. Mao Z, Fleming J, Mayans O, Frey J, Schleheck D, Schink B, Müller N. (2023). AMP-Dependent Phosphite Dehydrogenase (ApdA),
a Novel Phosphorylating Enzyme in Dissimilatory Phosphite Oxidation. PNAS. In press.

25. Stehle J, Fleming JR, Bauer PM, Mayans O, Drescher M. (2023) Titin UN2A acts as a stable, non-polymorphic scaffold in its binding to CARP. Chembiochem. e202300408.

doi.org/10.1002/cbic.202300408. (Epub ahead of print).

24. (preprint) Lambrecht R, Delgado ME, Gloe V, Schuetz K, Plazzo AP, Franke B, Phan TS, Fleming J, Mayans O, Brunner T. (2023) Liver Receptor Homolog-1 (LRH-1/NR5A2) orchestrates hepatic inflammation and TNF-induced cell death. BioRxiv. 2023.05.24.542039. 

doi.org/10.1101/2023.05.24.542039

23.      Fleming JR, Hauth F, Hartig JS, Mayans O. (2023). Crystal structure of a GCN5-related N-Acetyltransferase from Lactobacillus curiae. Acta Cryst F. In Press.

22.      Fleming JR, Müller I, Zacharchenko T, Diederichs K, Mayans O. (2023). Molecular insights into titin’s A-band. J Muscle Res & Cell Mot. 

doi.org/10.1007/s10974-023-09649-1.  (On-line ahead of print)

21.      Stergiou C, Williams R, Fleming JR, Zouvelou V, Ninou E, Andreetta F, Rinaldi E, Simoncini O, Mantegazza R, Bogomolovas J, Tzartos J, Labeit S, Mayans O*, Tzartos S*. (2023). Immunological and Structural Characterization of the Titin MIR Locus; Identification of the Middle MIR Domain, I110, as the Main Target of Titin Antibodies in Myasthenia Gravis. Biomedicines. 11(2), 449.

doi.org/10.3390/biomedicines11020449

20.      Sinn M , Stanoppi M , Hauth F, Fleming JR, Funck D, Mayans O, and Hartig JS. (2022). Guanidinoacid hydrolase activity of the human enzyme annotated as agmatinase. Scientific Rep. 2022 Dec 21;12(1):22088.

doi.org/10.1038/s41598-022-26655-4

19.      Su K, Mayans O, Diederichs K, Fleming JR[CA], (2022) PaSiMap: Pairwise sequence similarity mapping with PaSiMap: reclassification of immunoglobulin domains from titin as case study. Computational and Structural Biotechnology Journal. 20:5409-5419.

doi.org/10.1016/j.csbj.2022.09.034.

18.        Funck D&, Sinn M&, Fleming JR, Stanoppi M, Dietrich J, Mayans O, and Hartig JS (2022) Guanidine hydrolase is a novel Ni2+-dependent enzyme from the arginase family. Nature.  603(7901):515-521.

doi.org/10.1038/s41586-022-04490-x

17.        Bogomolovas J&, Fleming JR&, Franke B, Manso B, Simon B, Gasch A, Markovic M, Brunner T, Knöll R, Chen J, Labeit S, Scheffner M, Peter C and Mayans O (2021) Titin kinase ubiquitination aligns autophagy receptors with mechanical signals in the sarcomere. EMBO Rep. 22(10):e48018.

doi.org/10.15252/embr.201948018

16.        Stronczek C, Lange S, Bullard B, Wolniak S, Börgeson E, Mayans O and Fleming JR [CA]. (2021) The N2A region of titin has a unique structural configuration. J Gen Physiol. 153(7) e202012766.  

doi.org/10.1085/jgp.202012766

15.        Zhou T, Fleming JR, Lange S, Hessel AL, Bogomolovas J, Stronczek C, Grundei D, Ghassemian M, Biju A, Börgeson E, Bullard B, Linke WA, Chen J, Kovermann M and Mayans O. (2021) Molecular Characterisation of Titin N2A and Its Binding of CARP Reveals a Titin/Actin Cross-linking Mechanism. J Mol Biol. 433(9):166901. 

doi.org/10.1016/j.jmb.2021.166901

14.        Fleming JR [CA], Rani A, Kraft J, Zenker S, Börgeson E, and Lange S. (2021) Exploring Obscurin and SPEG Kinase Biology. Journal of Clinical Medicine. 10 (5), 984.

doi.org/10.3390/jcm10050984

13.       Fleming JR [CA], Rigden DJ, and Mayans O. (2020) The importance of chain context in assessing small nucleotide variants in titin: in silico case study of the I10-I11 tandem and its arrhythmic right ventricular cardiomyopathy linked position T2580. J Biomol Struct Dyn. 22, 1-11.

doi.org/10.1080/07391102.2020.1768148

 

12.       Nesterenko Y&, Hill CJ&, Fleming JR&, Murray P and Mayans O. (2019) The ZT biopolymer: a self-assembling protein scaffold for stem cell applications. International Journal of Molecular Sciences. 20(17), 4299.

doi.org/10.3390/ijms20174299

11.       Hill CJ, Fleming JR, Mousavinejad M, Nicholson R, Tzokov SB, Bullough PA, Bogomolovas J, Morgan MR and Mayans O. (2019) Self-Assembling Proteins as High-Performance Substrates for Embryonic Stem Cell Self-Renewal. Adv Mater. 31(17):e1807521.

doi.org/10.1002/adma.201807521

10.       Adams M, Fleming JR, Riehle E, Zhou T, Zacharchenko T, Markovic, M and Mayans O. (2019) Scalable, non-denaturing purification of phosphoproteins using Ga3+-IMAC: N2A and M1M2 titin components as study case. Protein Journal. 38(2):181-189.

doi.org/10.1007/s10930-019-09815-w

9.         Fleming JR, Schupfner M, Busch F, Baslé A, Ehrmann A, Sterner R, Mayans O. (2018) Evolutionary Morphing of Tryptophan Synthase: Functional Mechanisms for the Enzymatic Channeling of Indole. J Mol Biol. 430(24):5066-5079.

doi.org/10.1016/j.jmb.2018.10.013

8.         Williams RM, Franke, B, Wilkinson M, Fleming JR, Benian GM, Rigden D, Eyers PA and Mayans O. (2018) Autophosphorylation Is a Mechanism of Inhibition in Twitchin Kinase. J Mol Biol. 430(6):793-805.

doi.org/10.1016/j.jmb.2018.01.020

7.         Bogomolovas J, Fleming J, Anderson BR, Williams R, Lange S, Simon B, Khan MM, Rudolf R, Bullard B, Rigden DJ, Granzier H, Labeit S, Mayans O. (2016) Exploration of the pathological potential of isolated mSNPs in titin: the cardiomyopathy-linked mutation T2580I. Open Biology. 6(9): 160114.

doi.org/10.1098/rsob.160114

6.         Fleming JR&, Sastry L&, Napier GB, Sullivan L and Ferguson MA. (2016) Proteomic identification of immunodiagnostic antigens for Trypanosoma vivax infections in cattle and generation of a proof-of-concept lateral flow test diagnostic device. PLoS Negl Trop Dis.10(9):e0004977.

doi.org/10.1371/journal.pntd.0004977

5.         Zhou T&, Fleming JR&, Franke B, Bogomolovas J and Mayans O. (2016) CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex. FEBS Lett 590(18):3098-110.

doi.org/10.1002/1873-3468.12362.

4.         Fleming JR&, Sastry L&, Crozier TW, Napier GB, Sullivan L and Ferguson MA. (2014) Proteomic selection of immunodiagnostic antigens for Trypanosoma congolense. PLoS Negl Trop Dis. 8, e2936.

doi.org/10.1371/journal.pntd.0002936.

3.         Sullivan L, Fleming J, Sastry L, Mehlert A, Wall SJ and Ferguson MA. (2014) Identification of sVSG117 as an immunodiagnostic antigen and evaluation of a dual-antigen lateral flow test for the diagnosis of human African trypanosomiasis. PLoS Negl Trop Dis 8, e2976.

doi.org/10.1371/journal.pntd.0002976

2.         Fleming JR, Morgan RE, Fyfe PK, Kelly SM and Hunter WN. (2013) The architecture of Trypanosoma brucei tubulin-binding cofactor B and implications for function. FEBS J. 280 (14): 3270-80.

doi.org/10.1111/febs.12308

1.         Fleming JR, Dawson A and Hunter WN. (2010) Crystal structure of Leishmania major ADP-ribosylation factor-like 1 and a classification of related GTPase family members in this Kinetoplastid. Mol Biochem Parasitol. 174, 141-4.

doi.org/10.1016/j.molbiopara.2010.08.002.

Book chapters

(Reprint): Nesterenko Y, Hill CJ, Fleming JR, Murray P, Mayans O. (2020) “The ZT Biopolymer: A Self-Assembling Protein Scaffold for Stem Cell Applications.” In Designer Biopolymers: Self-Assembling Proteins and Nucleic Acids. Edited by Ayae Sugawara-Narutaki and Yukiko Kamiya. Published by MDPI. ISBN 978-3-03936-370-4 (Hbk); ISBN 978-3-03936-371-1; doi.org/10.3390/ijms20174299.

Protein Data Bank Entries

  1. 2X77 - Crystal Structure of Leishmania major ADP ribosylation factor-like (2010) doi.org/10.2210/pdb2X77/pdb
  2. 4B6W - Architecture of Trypanosoma brucei Tubulin-Binding cofactor B (2012) doi.org/10.2210/pdb4B6W/pdb
  3. 4B6M - Trypansoma brucei tubulin binding cofactor B CAP-Gly domain (2012) doi.org/10.2210/pdb4B6M/pdb
  4. 5JOE - Crystal structure of I81 from titin (2016) doi.org/10.2210/pdb5JOE/pdb
  5. 6EQN - Sulfolobus solfataricus Tryptophan Synthase B2o (2017) doi.org/10.2210/pdb6EQN/pdb
  6. 5N2P - Sulfolobus solfataricus Tryptophan Synthase A (2017) doi.org/10.2210/pdb5N2P/pdb
  7. 6HTE - Sulfolobus solfataricus Tryptophan Synthase B2a (2018)
    doi.org/10.2210/pdb6HTE/pdb
  8. 6FWX - Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin (2018) http://https://doi:10.2210/pdb6FWX/pdb
  9. 6HUL - Sulfolobus solfataricus Tryptophan Synthase AB Complex (2018)
    doi.org/10.2210/pdb6HUL/pdb
  10. 6SDB - Chimeric titin Z1Z2 functionalized with a KLER exogenous peptide from decorin (2019) doi.org/10.2210/pdb6SDB/pdb
  11. 7NIP -  titin N2A unique sequence (UN2A) core (2021) https://doi.org/10.2210/pdb7NIP/pdb
  12. 7AHS - titin-N2A Ig81-Ig83 (2021) https://doi.org/10.2210/pdb7AHS/pdb
  13. 7ESR - Crystal structure of Synechocystis sp PCC6803 guanidinium hydrolase (R32) https://doi.org/10.2210/pdb7ESR/pdb
  14. 7OI1 - Crystal structure of Synechocystis sp PCC6803 guanidinium hydrolase https://doi.org/10.2210/pdb7OI1/pdb
  15. 8BVO -  Titin I110-I111 FnIII tandem from the MIR region (I/A5-I/A6)
  16. 8BXR -  Titin FnIII-domain I109-I111 (I/A4-A/A6) from the MIR region
  17. 8BW6 -   Titin FnIII-domain I110 (I/A6) from the MIR region
  18. 8BNQ - Crystal structure of the FnIII-tandem A84-A86 from the A-band of titin
  19. 8OSP - GCN5-related N-Acetyltransferase from Lactobacillus curiae